Carabid beetle genome #seqthebeetle
This seemed fitting given our current bombardier beetle expedition. In addition, our very own CalBug data provider and NfN founder member Kip Will is a principal investigator on the project.

Help get a genome for the amazing Bombardier beetle #seqthebeetle
Vote daily from now until 5 April here https://tinyurl.com/gn84mu8
Phenology of Oaks: A recap
A huge shout out to our volunteers for quick work on our first NFN Ideas project, which focused on oak phenology. We completed the expedition a week after launching it, with 1944 transcripts of 644 subjects. 53 awesome transcribers took part. A lot of discussion on talk focused on some of the challenges with denoting flowers and fruits — it is harder than it first looks! So folks were interested in whether there was consistency among transcribers, and if the results would be consistent with an expert assessment. We have some initial answers to those questions and more! And a note that ALL of these data – the label data and phenological scoring – were ALL done by Notes from Nature volunteers.
So to get right to it! Transcriber consistency on this expedition was absolutely remarkable. Well above 99%. Yeah. We were surprised, too. There were three cases where we didn’t get consistent results. Just 3! Out of 664 subjects. So apparently there was very strong agreement.
We took a closer look at the three that seemed to prove difficult.
Those were:
- subject_id: 4308678 –http://www.sernecportal.org/portal/collections/individual/index.php?occid=11108535
- subject_id: 4308659 –http://www.sernecportal.org/portal/collections/individual/index.php?occid=11108069
- subject_id: 4308844 —http://www.sernecportal.org/portal/collections/individual/index.php?occid=11130030
The consensus scoring for those from transcribers were:
- subject_id: 4308678: Flowers: No, Fruits: No
- subject_id: 4308659: Flowers: No, Fruits: Yes
- subject_id: Flowers: Yes, Fruits: No
I then asked NFN’s own Michael Denslow, who is also a darn fine botanist, for his assessment (without reporting anything about transcriber’s scoring), and he was 100% consistent with the three above. He noted for 4308678, “Funky one for sure” and for 4308659, “The terminal buds might be confusing people on these. Based on the collection date (and presence of terminal buds) fruits could be from pervious fall.”

And finally, we wanted to see if we could use these data to look at phenology patterns, so our data scientist Julie Allen did some quick visualizations of the data using the statistical package, R, which has some great plotting functions. You can see our plot above, for two species, Quercus falcata (top) and Quercus marilandica (bottom), two common oaks where we had enough data to examine patterns. The plot shows time on x-axis measured from March through November, and the y-axis is just a yes-no response. For yesses, we show a little emoji, and for no’s you can see those no reports over time for fruits and flowers in different colors. Yup, we decided to go with a tropical flower and fruit motif here, despite oaks definitively not producing pineapples!
The really neat thing is that we do pick up the short, and early flowering period for oaks during Spring, and in Q. falcata, a seemingly quick transition to acorns, and a slower cadence for Q. marilandica (note the longer period between flowering and appearance of acorns). There are still some great questions to examine here — these records were not all from the same year, and maybe some variation we are seeing is due to climate variation year to year. There were a couple “no flower” records during a typical flowering period and these might be either limited information from the sample, or perhaps something about that particular year. We are more than happy to share the raw data from this expedition with anyone who wants a closer look!
Leafcutter bees: building the perfect home
Many people have heard of leafcutter ants carrying bits of leaves around the forest like little parasols. Ants are all in the family Formicidae, in the order Hymenoptera, which includes bees, wasps, and ants. Leafcutter ants do not eat the leaves, but rather compost them to feed their fungus gardens. They only eat a particular species of fungus, removing all other species, and constantly tend their gardens to keep them clean.

Photo: Peter Oboyski

Photo: jacinta lluch valero https://flic.kr/p/ne1WHh
New Bombardier beetle expedition
Thanks to everyone who contributed to the Trechine Ground Beetles 2 expedition. With its completion, we have also finished off our remaining specimens from this ground beetle subfamily, and brought our total number of ground beetle specimens digitized to 4391. That’s a little less than 25% of the total number of specimens we had when we started this project back in July, 2016.
For our next expedition we’ll be focusing on the Bombardier beetles (subfamily Brachininae). The University of Alberta’s E.H. Strickland Entomological Museum has a large number of Bombardier beetle specimens, with 860 already digitized and publicly available from GBIF, and another ~4300 with images remaining to be transcribed as part of this effort. Most of these belong to just one genus, Brachinus, though there are about a dozen genera known in the subfamily. Bombardier beetles are found in temperate and subtropical regions around the world. They get their name from their ability to spray boiling hot chemicals from their abdomen, a characteristic whose mechanical/biological basis eluded explanation until a couple of years ago (a must see article and video here).
You’ll notice that our expedition image has changed from what use to be a photo of a specimen drawer to something a little more lively. We now feature an in situ photograph of a Brachinus specimen courtesy of PhD student and carabidologist, Wesley Hunting. Thanks Wes!
Make sure to check out our Bombardier beetle expedition!
Cheers,
–Bryan Brunet, PhD
Collections Management Advisor (Natural Sciences), University of Alberta Museums, Edmonton, Alberta, Canada
The Dentist who collected Fairyflies
For those of you who have been helping us to transcribe the Chalcid specimen labels in Miniature Lives Magnified, you’ll have been coming across one particular style of slide label that always get us flipping our heads from side to side to read.
And almost all of those specimens seem to have been collected in Bournemouth, and the surrounding area.
Once you start seeing a few of these, you’ll start to notice that they are all marked ‘S.B.’ – who is in fact the Collector of these specimens.
I started to become curious about the mysterious S. B., and the hyper-local nature of his or her collecting, so I decided to do a bit of sleuthing with the help of our Curator Natalie.
“I do know about the Bournemouth man: he was a dentist, and originally a lepidopterist but worked a lot on Mymarids… “
So here’s what I’ve found out.
S.B. is Sidney Charles Scarsdale Brown – born in London in 1903, and passed away in Bournemouth, Dorset in 2003.
You will have noticed that many of his slides are also marked ‘Trap‘ (which is why we don’t see host insect & host plant information on these). Mr. Brown has written something about how he found these marvelous creatures in the following note in the Entomologist’s Monthly Magazine: 108: 94 (1973):

Mr Brown is mentioned on page 18 of The Conservation of Invertebrates report of the Monks Wood Experimental Station Symposium No 1, March 23rd – 25th, 1965:
“…a great deal of the information about the insect fauna of Scotland can be found in the diaries of a Mr. Harwood who lived in Aviemore just before the War…the diaries were now in the possession of Mr. Scarsdale Brown of Bournemouth, and on his death would be handed to the Hope Department, Oxford.”

Mymaridae – Fairy Fly – Stephanodes elegans
He is also listed with all of his bona fides as S.C.S. Brown. F.D.S., L.D.S.. R.C.S., Vice President of the Bournemouth Natural Science Society, in their published proceedings from 1989 – 1990, which shares this wonderful background information about him:
“At the present time, the Society is very fortunate to have as a member Mr S.C. Scarsdale Brown. He joined the Society in 1937, was Chairman of Entomology 1939- 48.
After a few years away from the Society, he rejoined in 1967 and was – 44 – President 1975-76, the subject of his Presidential Address being “The Natural History of Bournemouth 1800-1900”. He edited the Proceedings from 1978-82 and is now an Honorary Member.
He has lived all his life in the Bournemouth area, working as a Dental Practitioner.
As a young man his interests centred on the Macrolepidoptera. He met W. Parkinson Curtis, who became a life-long friend, and joined the Society for British Entomology. There he met eminent entomologists such as Lt. Cdr. Fraser and William Fassnidge. The latter introduced him to the world of Microlepidoptera, at which he quickly became an expert, especially on the group of tiny moths known as Nepticulidae. Mr Brown was one of the contributors to the Illustrated Papers on British Microlepidoptera published in 1978 by the British Entomological and Natural History Society, his paper being illustrated with the superb paintings of Lt. Col. Fraser.
A meeting with Philip Harwood – one of the finest field entomologists – further added to his interests. Harwood concentrated on what are known to Lepidopterists as “Other Orders”, i.e. groups such as Coleoptera (beetles), Hemiptera (bugs) and Hymenoptera (bees and wasps).
Scarsdale Brown has studied all these groups, but has become one of the country’s leading specialists in Aculeate and Parasitic Hymenoptera. His meticulous and painstaking fieldwork is illustrated by his work on the group of Parasitic Hymenoptera known as Fairy Flies (family Mymaridae). This contains what must be some of the world’s smallest insects – one of the largest of the fairy flies has a wingspan of only 3mm; they pass their larval stages inside the eggs of other insects such as dragonflies.
During the course of his study, he has recorded 7 species of Mymaridae new to Britain.
His collection of specimens is a joy to behold – each fairy fly mounted in a microscope slide, and the tiny Nepticulid moths perfectly set. Mr Brown is an excellent artist in watercolour, and has contributed many notes to entomological magazines.
Over the years the members of the Society have heard some fascinating lectures from him on Hymenoptera and other groups. In 1988 he received an award in the Manse 1-P leydel 1 Prize Essay Competition.
In recent years he has suffered from failing eyesight, which has prevented any of the entomological study he loves, but he still attends some lectures of the Society, and maintains his own garden, where he specialises in growing camellias and lilies.”
We thought the timing was good for a phenology expedition

It’s tantalizing close to Spring here in North America, and some of you may have noticed hints of it, not just the nicer weather, but maybe moreso the first blooms of redbud, apple and plum and cherry trees, dogwoods, citrus. These harbingers of Spring have been part of not only the natural season cycle, but also human cultural history, for millennia.
People have been recording phenology for thousands of years. In Japan, records were kept of cherry blossoms back starting in 812AD. Yeah, not 1812, but 812. These have become part of national festivals around the world, with famous festivals in Tokyo and Washington DC. The date at which plants first flower, or have peak flowering, are often directly linked to climate conditions. The same is true of first appearance of new leaves, or new fruits. In Fall, leaf senescence, with first changes in leaf color, is also tied to temperature, as well as day length.
How do we know historical patterns of phenology? In some cases, we can refer to diaries and other written records, such as the case in historical record-keeping in Japan. In the last decade, we have a lot more tools at our disposal, including remote sensing – yes, imaging devices on satellites can tell when our planet gets more green! But for phenology records going back the last century, one powerful resource are natural history collections. A lot of the specimens collected and stored in museums tell us about phenology. But in order to use those data effectively for science, we need to actually report phenological stages and traits from those specimens. And that is where you come in!
Our newest expedition is a different beast than ones we have run in the past. In this expedition, you’ll help us determine if specimens from oak trees have flowers or fruits. These specimens already had their labels transcribed as part of an earlier expedition, and now we’ll annotate phenology information as well, and have that information associated with the specimen label data. These data can be used to look at historical patterns of phenology, and linked to past climate data records, to determine relationships, ultimately across many different species.
We are interested in your feedback on this first try at Notes from Nature with reporting phenology. In later expeditions, we may ask for more detailed information such as how many flowers are visible on a specimen, or fruits (in the case of oaks, we call the fruits “acorns”). For now, thanks for the help with enhancing the ability to use specimens for new kinds of science. We’ll share more about the science we are hoping to enable in later blog posts.
Finally, we are also launching our first mini-course with this expedition. This mini-course is about phenology, and how and why it is studied. What is a mini-course? Every 5 or so transcriptions, you’ll get some more information on phenology. We have 11 total “slides” in our mini-course, so you can see them all if you do 55 transcriptions. We haven’t tried a mini-course before, and we hope it proves interesting and not distracting. Let us know!
Introducing NFN Labs
We have always wanted to make Notes from Nature a place where our amazing volunteers can try out new activities, as long as they have high scientific value. In order to try out some of those activities, and find out what you think about them, we are excited to launch a new section of Notes from Nature called “NFN Labs”. The “Labs” idea lets us be experimental, while we remain committed to our core mission of helping to unlock natural history data, one record at a time.
NFN Labs is a place to try new kinds of expeditions. For example, we might have a labs expedition where instead of transcribing most of specimen label, we might have just one field that is needed. Or maybe we ask for help getting latitude and longitude information based on textual descriptions of localities where a specimen was collected. Or, as in the case of our first NFN Labs expedition, we might ask you to answer a question or two about the specimen itself, such as whether it has flowers or fruits. We also want to use the NFN Labs idea to test new features and get feedback. In the first Labs expedition, we are also launching our first mini-course. Mini-courses provide extra content on a topic that shows up after performing a certain number of transcriptions – in this case after 5 transcription. The topic of the first mini-course covers why phenology is important to study.
After we have tested out some labs ideas, they may move into more full “rotation” as part of Notes from Nature. We really hope some of these new experiments are cool and fun to do, while also keeping the same high bar of having immediate value for scientific use. We always appreciate (and crave) feedback. What do you think of our first NFN Lab?
Spectacular Underwing Moths III
We have a new expedition of Spectacular Underwing Moths! In this new expedition you will see familiar faces (species), but you will also see new ones. While it is hard to differentiate the new species from the old, there is one that will set itself apart: The Epione Underwing. The goal of this project is to look at the distribution of underwing moths over the last 100+ years. These data are needed to understand how distributions might have changed over time, and the only way to discover this is by transcribing specimen labels that are pinned to each specimen from the Florida Museum of Natural History’s McGuire Center.
It’s important to remember that you will be looking at two images per moth, dorsal and ventral. The reason for this is because there has been important information on the back of labels. So be sure to check both images! While checking both images, look at the amazing patterns, or lack of patterns, and the colors of the moth. The Epione Underwing does not have bright stripe markings, it has a solid-color hindwing. It might seem drab, but take a closer look at the moth’s ventral side, where a pattern resides. Obtaining these data from both sides of the label is very important for research and conservation of these beautiful moths, so please help!

— Stacey Huber, University of Florida
High five!
Thank you NfN citizen scientists for helping complete the second expedition digitizing the spectacular underwing moths! This expedition of 1000 images was a little tricky with some hard-to-read labels, but you all managed to power through. It is remarkable work. Thank you for taking the time to help. With this information, researchers can begin to examine the moths’ distribution changes, changes in host plants, and impact of climate change during the last century.
Be on the lookout for the next underwing moth expedition, coming very soon. The next expedition will have many new species. Maybe you will notice the differences!


